Advances in Applied Biotechnology: Proceedings of the 2nd by Tong-Cun Zhang, Motowo Nakajima

By Tong-Cun Zhang, Motowo Nakajima

At the ICAB 2014, researchers from worldwide will assemble to debate the most recent medical study, findings and applied sciences bearing on Microbial Genetics and Breeding, Optimization and keep an eye on of organic tactics, organic Separation and organic Purification, and Advances in Biotechnology.
This convention will supply a platform for tutorial trade at the program of biotechnology among family and overseas universities, examine institutes, company specialists and students. The members will concentrate on the overseas improvement and destiny developments. the development will lay a superb starting place for addressing key technical demanding situations in numerous components of utilized biotechnology, delivering possibilities to advertise the improvement and enlargement of the biotechnology industry.

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5 Optimization of Temperature and pH for Hydrolytic Activity of Recombinant Cassava β-Glucosidase The recombinant cassava β-glucosidase was sensitive to temperature and pH values. As shown in Fig. 5a, when the temperature increased from 10 to 35 °C, the activity increased gradually. The maximum activity was achieved at 35 °C. If the temperature continued to rise, the activity decreased seriously. 0 % of maximal activity. Fig. 5 Optimization of temperature and pH for hydrolytic activity of recombinant cassava β-glucosidase.

The PCR program consisted of predenaturation at 95 °C for 8 min, denaturation at 95 °C for 30 s, annealing at 57 °C for 1 min, and extension at 72 °C for 10 min for a total of 32 cycles. The genome of B. cereus was carried out with a DNA extraction kit from CWBIO (Beijing, China). The genome of C. boidinii was obtained as described by Manuel et al. [7]. 1. 3 Construction of pLeuDHFDH Plasmid for the simultaneous expression of LeuDH and FDH (pLeuDHFDH) was constructed as shown in Fig. 2. 2). The LeuDH gene was cloned by PCR by using pLeuDH as template and was introduced into pET-28a at the BamHI and SacI sites, and then the FDH gene was cloned by PCR by using pFDH as template and was introduced into the plasmid at the SalI and XhoI sites to produce plasmid pLeuDHFDH (Fig.

Wandrey C, Bossow B (1986) Continuous cofactor regeneration-utilisation of polymer bound NAD (H) for the production of optically active acids. Biotechnol Bioind 3:813 5. Ohshima T, Soda K (1989) Thermostable amino acid dehydrogenases: applications and gene cloning. Trends Biotechnul 7:210–214 6. Bommarius AS, Schwarm M, Sfingl K, Kottenhahn M, Huthmacher K, Drauz K (1995) Synthesis and use of enantiomerically pure tert-leucine. Tetrahedron Asymmetry 6:2851–2888 7. Manuel RK, Luis ES, Ericka PF, Anne G (2011) Strategy for the extraction of yeast DNA from artisan agave must for quantitative PCR analysis.

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